Curation Information

Publication

Dual negative control of spx transcription initiation from the P3 promoter by repressors PerR and YodB in Bacillus subtilis.;Leelakriangsak M, Kobayashi K, Zuber P;Journal of bacteriology 2007 Mar; 189(5):1736-44 [17158660]

TF

YodB [O34844, view regulon]

Reported TF sp.

Bacillus subtilis JH642

Reported site sp.

Bacillus subtilis JH642

Created by

Grace Chandler

Curation notes

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Experimental Process

A spx:bgaB fusion assay demonstrated repression of spx by YodB. Primer extension and In vitro transcription assays validated this claim. Site directed mutagenesis of the upstream region of spx resulted in derepression. DNase I footprinting localized the binding site. Site directed mutagenesis of this identified site confirmed its role in repression.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.