Curation Information

Publication

Involvement of the leucine response transcription factor LeuO in regulation of the genes for sulfa drug efflux.;Shimada T, Yamamoto K, Ishihama A;Journal of bacteriology 2009 Jul; 191(14):4562-71 [19429622]

TF

LeuO [P10151, view regulon]

Reported TF sp.

Escherichia coli str. K-12 substr. W3110

Reported site sp.

Escherichia coli str. K-12 substr. W3110

Created by

Dinara Sagitova

Curation notes

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Experimental Process

LeuO binding sequences in 12 regions on the E. coli genome were identified by genomic SELEX. LeuO binding to SELEX fragments was confirmed by EMSA. LeuO-dependent expression of the its putative target genes was confirmed by Northern blot analysis in the wild-type E. coli BW25113 and its isogenic leuO mutant.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome
• Site-specific quantitative values

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.