Curation Information

Publication

The LysR-type transcriptional regulator LeuO controls expression of several genes in Salmonella enterica serovar Typhi.;Hernández-Lucas I, Gallego-Hernández AL, Encarnación S, Fernández-Mora M, Martínez-Batallar AG, Salgado H, Oropeza R, Calva E;Journal of bacteriology 2008 Mar; 190(5):1658-70 [18156266]

TF

LeuO [Q8Z9H8, view regulon]

Reported TF sp.

Salmonella enterica subsp. enterica serovar Typhi str. CT18

Reported site sp.

Salmonella enterica subsp. enterica serovar Typhi str. CT18

Created by

Dinara Sagitova

Curation notes

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Experimental Process

LeuO-regulated proteins were identified by MALDI-TOF Mass Spectrometry. CAT reporter assays showed that LeuO activated the STY3070-STY3064 operon transcription and repressed the expression of tpx, ompX, and STY1978. Primer extension analysis was used to determine the transcription start sites of LeuO-regulated genes. DNase I footprinting determined the binding regions involved in LeuO-dependent regulation of STY3070, assT, ompS1, ompS2, tpx, ompX, and STY1978. The authors employed pattern discovery algorithms to identify LeuO consensus binding site and reported that LeuO binding sites are not sequence specific and may not be easily represented with a consensus.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.