Curation Information

Publication

A novel DNA-binding site for the ferric uptake regulator (Fur) protein from Bradyrhizobium japonicum.;Friedman YE, O'Brian MR;The Journal of biological chemistry 2003 Oct 3; 278(40):38395-401 [12881516]

TF

Fur [H7C6Q1, view regulon]

Reported TF sp.

Bradyrhizobium diazoefficiens USDA 110

Reported site sp.

Bradyrhizobium diazoefficiens USDA 110

Created by

Grace Chandler

Curation notes

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Experimental Process

EMSA was used to demonstrate Fur binding to the irr promoter. DNase I footprinting localized the Fur binding region in the irr promoter. In vitro transcription assays confirmed repression of irr expression by Fur.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.