Curation Information

Publication: Cell cycle-dependent transcriptional and proteolytic regulation of FtsZ in Caulobacter.;Kelly AJ, Sackett MJ, Din N, Quardokus E, Brun YV;Genes & development 1998 Mar 15; 12(6):880-93 [9512521]
TF: CtrA [P0CAW8, view regulon]
Reported TF sp.: Caulobacter crescentus NA1000
Reported site sp.: Caulobacter crescentus NA1000
Created by: Dinara Sagitova
Curation notes: -

Experimental Process

Visual inspection of the ftsZ promoter identified a sequence with the perfect match to the CtrA consensus binding site. DNase I footprinting confirmed that CtrA binds to the predicted binding site. ftsZ-xylX fusion assays using ftsZ promoter in which CtrA binding site was deleted showed that ftsZ expression was 1.7-fold higher than in the wild-type strain.

Transcription Factor Binding Sites

Sites reported in the paper
Sites matched in the genome

TTAACCGCCGATTAAC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence	Regulated genes	Gene diagram	Experimental techniques	TF function	TF type
TTAACCGCCGATTAAC	ftsZ,		Experimental technique details DNAse footprinting (ECO:0005631) DNAse footprinting - ECO:0005631 The DNAse foot-printing method starts by focusing on a given region of interest (e.g. a promoter region) and amplifying it by PCR to obtain lots of sample. It then throws in the TF and then the DNAse. The mix is left to stir for a short time and then gel electrophoresis is run to compare the pattern of fragments in a control (no TF) and in the sample. If the TF has bound the sample, it will have protected a stretch of DNA (encompassing some fragments of the control) and thus those fragments will not appear in the sample gel. The fragments can then be cut-out from the gel, purified and sequenced to obtain the sequence of the protected region. This is often used to identify the binding motif of a TF for the first time. The foot-printing will typically resolve the protected region down to 50-100 bp, and the sequence can be then examined for possible TF-binding sites either by eye of using a computer search. - Experimental technique details Visual sequence inspection (nan) Visual sequence inspection - nan Visual inspection of promoter region to identify putative binding sites based on previous knowledge - Experimental technique details xylE reporter assay xylE reporter assay A reporter assay based on the fusion of a promoter of interest with the xylE gene, which produces catechol dioxygenase, capable of converting the colorless substrate catechol to yellow hydroxymuconic semialdehyde. -	repressor	not specified