Site information for: Phd in species Salmonella enterica; genome accession: NC

Transcription Factor Binding Site Information

dbxref: CollecTF:EXPSITE_0002cc00
Genome: Salmonella enterica - NC_003197.1
TF: Phd [UniProtKB:P40819, view regulon]

Supporting Evidence

Binding site	Location	Publication	Experimental techniques used	Curation
TTGTACAA	- [3728550, 3728557]	15743936	Experimental technique details Beta-gal reporter assay Beta-gal reporter assay Reporter assay using the beta-galactosidase (lacZ) gene. The lacZ gene is typically fused to the promoter of interest. Differential regulation of the promoter mediated by the TF is assessed by induction of the system and evaluation of lacZ expression. Bacteria expressing lacZ appear blue when grown on a X-gal medium. The assay is often performed using a plasmid borne construction on a lacZ(def) strain. - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) Multiple sequence alignment (MSA) - ECO:0005556 MSA can be used to align promoter regions and highlight regions of conservation that may be indicative of transcription factor binding sites. -	1039

Regulated genes

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Gene	Locus tag	Description
yhhV	STM3559	hypothetical protein
STM3558	STM3558	death-on-curing protein