Curation Information

Functional roles of arcA, etrA, cyclic AMP (cAMP)-cAMP receptor protein, and cya in the arsenate respiration pathway in Shewanella sp. strain ANA-3.;Murphy JN, Durbin KJ, Saltikov CW;Journal of bacteriology 2009 Feb; 191(3):1035-43 [19060154]
CRP [A0KST7, view regulon]
Reported TF sp.
Shewanella sp. ANA-3
Reported site sp.
Shewanella sp. ANA-3
Created by
Grace Chandler
Curation notes

Experimental Process

qRT-PCR demonstrated CRP acts an activator of arr expression. A PSSM was generated based off of E.coli CRP binding motifs, with higher scores signaling putative binding sites. EMSA confirmed binding of CRP to the arr promoter.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
CTATTTGCTACTGATCACATTT Shewana3_2342, Shewana3_2343, Shewana3_2344, Shewana3_2345,
... ... Shewana3_2342 Shewana3_2343 Shewana3_2344 Shewana3_2345 Shewana3_2341 Shewana3_2340
Experimental technique details EMSA (ECO:0001807) - Experimental technique details PSSM site search (ECO:0005659) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - activator not specified