Curation Information

Identification of direct transcriptional target genes of ExoS/ChvI two-component signaling in Sinorhizobium meliloti.;Chen EJ, Fisher RF, Perovich VM, Sabio EA, Long SR;Journal of bacteriology 2009 Nov; 191(22):6833-42 [19749054]
ChvI [P50350, view regulon]
Reported TF sp.
Sinorhizobium meliloti 1021
Reported site sp.
Sinorhizobium meliloti 1021
Created by
Joe Sparenberg
Curation notes

Experimental Process

Site directed mutagenesis was used to create a strain with increased chvI function. Microarray analysis discovered that 55 genes were activated by ChvI and 4 genes are repressed by ChvI. GUS reporter gene assay was used on 7 of those genes (ropB1, SMb21188, SMb21440, SMb21491, SMc01580, SMc01855, SMc00159) and the results validated the results obtained from the microarray analysis. EMSA determined direct binding upstream of the SMc01580 gene. Direct binding was also observed upstream of the ropB1 gene and the SMb21440. DNase I footprinting localized the region bound by ChvI in the SMc01580 promoter. MEME algorithm identified an 11-bp-long motif that was present at least once in each of the ChvI-regulated genes from the microarray analysis

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... SMc01580 aatA SMc01581 SMc01583
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details GUS reporter gene assay (ECO:0005641) - activator not specified