Curation Information

SO2426 is a positive regulator of siderophore expression in Shewanella oneidensis MR-1.;Henne KL, Wan XF, Wei W, Thompson DK;BMC microbiology 2011 May 31; 11(1):125 [21624143]
SO2426 [Q8EEF6, view regulon]
Reported TF sp.
Shewanella oneidensis MR-1
Reported site sp.
Shewanella oneidensis MR-1
Created by
Grace Chandler
Curation notes

Experimental Process

In silico searches using the tools MEME, MotifSampler & Gibbs Recursive sampler on previously collected microarray data, developed a consensus binding motif for SO2426 and identified potential binding sites. EMSA confirmed binding of SO2426 to the siderophore expression operon so3030-3031-3032. Quantitative phenotypic analysis of siderophore production showed lower expression in SO2426 deletion mutants, confirming that SO2426 acts as an activator.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... pubA pubB pubC putA putB
Experimental technique details Ad-hoc quantitative phenotype observation (ECO:0005676) - Experimental technique details Consensus search (ECO:0005624) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Motif-discovery (ECO:0005558) - activator not specified