Curation Information

Rem, a new transcriptional activator of motility and chemotaxis in Sinorhizobium meliloti.;Rotter C, Mühlbacher S, Salamon D, Schmitt R, Scharf B;Journal of bacteriology 2006 Oct; 188(19):6932-42 [16980496]
Rem [F7X8H2, view regulon]
Reported TF sp.
Sinorhizobium meliloti Ru11/001
Reported site sp.
Sinorhizobium meliloti Ru11/001
Created by
Grace Chandler
Curation notes

Experimental Process

Western blot analysis and lacZ fusion assays demonstrated auto repression by Rem. These results were validated by growth curve analysis. DNase I footprinting of their promoters revealed a putative Rem binding site. Multiple sequence alignment of this site and other related promoters identified a Rem consensus binding sequence.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... rem flgE flgK flgL flaF flbT flgD fliQ flhA fliR SM11_chr0327 SM11_chr0328 SM11_chr0329 SM11_chr0330
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details Growth curve analysis (ECO:0005638) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details Western blot (quantitative) expression analysis (ECO:0000279) - repressor not specified