Curation Information

Vibrio cholerae VibF is required for vibriobactin synthesis and is a member of the family of nonribosomal peptide synthetases.;Butterton JR, Choi MH, Watnick PI, Carroll PA, Calderwood SB;Journal of bacteriology 2000 Mar; 182(6):1731-8 [10692380]
Fur [A5F6G4, view regulon]
Reported TF sp.
Vibrio cholerae O395
Reported site sp.
Vibrio cholerae O395
Created by
Dinara Sagitova
Curation notes

Experimental Process

vibF-lacZ reporter assays showed that vibF was negatively regulated by Fur in the presence of high iron concentration. Primer extension analysis localized the vibF transcription start site. Visual sequence inspection identified a putative Fur binding site homologous to the E.coli Fur consensus. DNase I footprinting confirmed that Fur binds to its target operator site in the vibF promoter in the presence of iron.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... vibF
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details Visual sequence inspection (nan) - repressor not specified