vibF-lacZ reporter assays showed that vibF was negatively regulated by Fur in the presence of high iron concentration. Primer extension analysis localized the vibF transcription start site. Visual sequence inspection identified a putative Fur binding site homologous to the E.coli Fur consensus. DNase I footprinting confirmed that Fur binds to its target operator site in the vibF promoter in the presence of iron.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|