Curation Information

The LysR-type PcaQ protein regulates expression of a protocatechuate-inducible ABC-type transport system in Sinorhizobium meliloti.;Maclean AM, Haerty W, Golding GB, Finan TM;Microbiology (Reading, England) 2011 Sep; 157(Pt 9):2522-33 [21700663]
PcaQ [Q92TL9, view regulon]
Reported TF sp.
Reported site sp.
Sinorhizobium melloti RmP110
Created by
Grace Chandler
Curation notes

Experimental Process

Site-directed mutagenesis of this site, in conjunction with GFP reporter assays validated the specificity of PcaQ binding, and the activation of pcaM by PcaQ. Beta-gal assays and GFP assays both verified this activation of pcaM. EMSA confirmed PcaQ binding to the pcaM promoter region.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... SM_b20568 SM_b20787 SM_b20786 SM_b20785 SM_b20784
Experimental technique details Beta-gal reporter assay - Experimental technique details Comparative genomics search (ECO:0005622) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details GFP Promoter Fusion (ECO:0005636) - Experimental technique details Site directed mutagenesis (ECO:0005667) - activator not specified