Curation Information

Publication
FixJ-regulated genes evolved through promoter duplication in Sinorhizobium meliloti.;Ferrières L, Francez-Charlot A, Gouzy J, Rouillé S, Kahn D;Microbiology (Reading, England) 2004 Jul; 150(Pt 7):2335-45 [15256575]
TF
FixJ [P10958, view regulon]
Reported TF sp.
Sinorhizobium meliloti 1021
Reported site sp.
Sinorhizobium meliloti 1021
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

22 regions with affinity for phosphorylated FixJ were identified by genomic SELEX. Eight of these regions were confirmed by DNase I footprinting analysis. Promoter-GUS reporter assays determined that only two of these genes (smc03253, proB2) appeared to be FixJ-regulated. The authors report that smc03253 follows two FixJ binding sites, which share extensive homology with the pfixK region located on pSymA.

Transcription Factor Binding Sites


CTAGGTAGTTTCCC
CTAGGTAGTTTCCC
CTAGGTAGTTTCCC
CTAGGTAGTTTCCC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
CTAGGTAGTTTCCC SMc03253
... ... SMc03253
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details Genomic SELEX (ECO:0005634) - Experimental technique details GUS reporter gene assay (ECO:0005641) - activator not specified
CTAGGTAGTTTCCC SMc03253
... ... SMc03253
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details Genomic SELEX (ECO:0005634) - Experimental technique details GUS reporter gene assay (ECO:0005641) - activator not specified