22 regions with affinity for phosphorylated FixJ were identified by genomic SELEX. Eight of these regions were confirmed by DNase I footprinting analysis. Promoter-GUS reporter assays determined that only two of these genes (smc03253, proB2) appeared to be FixJ-regulated. The authors report that smc03253 follows two FixJ binding sites, which share extensive homology with the pfixK region located on pSymA.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|