IscR was found to be essential for Y. pseudotuberculosis virulence in an oral infection model performed in mice. RNAseq analysis identified differentially-regulated genes in the iscR mutant relative to the wild-type Y. pseudotubeculosis strain. qRT-PCR validated the results obtained by RNAseq for selected genes. Position specific scoring matrix was generated by the alignment of the known E. coli IscR type 2 motifs. PSSM search identified two IscR type I motifs in the iscRSUA operon.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|
|ATAGTTGAGTGAATTACTAGGTTAA||YPTB2860, iscS, nifU||
|ATAGTTGACTAAAACACTCAAGAAT||YPTB2860, iscS, nifU||