A pla-lacZ fuison determined CRP activates pla expression. Visual inspection of the pla upstream region revealed a sequence similar to the CRP binding motif in E. coli. EMSA confirmed binding to the pla upstream region. DNase I footprinting localized this binding site. Site-directed mutagenesis in conjunction with pla-lacZ fusion assays demonstrated the specificity of CRP binding.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|