Curation Information

LexA-independent DNA damage-mediated induction of gene expression in Myxococcus xanthus.;Campoy S, Fontes M, Padmanabhan S, Cortés P, Llagostera M, Barbé J;Molecular microbiology 2003 Aug; 49(3):769-81 [12864858]
LexA [Q1D406, view regulon]
Reported TF sp.
Myxococcus xanthus DK 1622
Reported site sp.
Myxococcus xanthus DK 1622
Created by
Erill Lab
Curation notes

Experimental Process

Beta-gal evidence of DNA-damaged induction of LexA. EMSA on multiple-sized fragments to validate binding. DNAse foot printing and site-directed mutagenesis to confirm site.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... lexA MXAN_4445 MXAN_4444 MXAN_4443 MXAN_4447 MXAN_4448
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor dimer