Curation Information

BswR controls bacterial motility and biofilm formation in Pseudomonas aeruginosa through modulation of the small RNA rsmZ.;Wang C, Ye F, Kumar V, Gao YG, Zhang LH;Nucleic acids research 2014 Apr; 42(7):4563-76 [24497189]
BswR [Q9I063, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Dinara Sagitova
Curation notes

Experimental Process

Analysis of the PrsmZ-directed b-gal. activity showed that deletion of bswR decreased the rsmZ expression, and overexpression of bswR increased the rsmZ expression. Consistent with these results, real-time RT-PCR assay also showed that the transcript level of rsmZ was decreased by 60% in the bswR-deleted mutant and increased by 3-fold in the bswR-overexpression strain. EMSA confirmed that BswR binds to the promoter region of rsmZ. DNase I footprinting assay identified the BswR binding region.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type