Analysis of the PrsmZ-directed b-gal. activity showed that deletion of bswR decreased the rsmZ expression, and overexpression of bswR increased the rsmZ expression. Consistent with these results, real-time RT-PCR assay also showed that the transcript level of rsmZ was decreased by 60% in the bswR-deleted mutant and increased by 3-fold in the bswR-overexpression strain. EMSA confirmed that BswR binds to the promoter region of rsmZ. DNase I footprinting assay identified the BswR binding region.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|