Visual inspection of the algD promoter identified two sites matching IHF consensus sites in E.coli. EMSA confirmed binding of IHF to the algD promoter. The author identified that mutations in each of the sites resulted in reduction or loss of in vitro IHF binding and a three- to fourfold decrease in algD-cat expression.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|