lac-Z assays showed that ohr expression is repressed by OhrR. GUS reporter gene assay showed equivalent results. Visual sequence inspection identified two palindromes resembling the OhrR binding site. EMSA results showed direct binding of OhrR to both motifs. Site directed mutagenesis in conjunction with EMSA confirmed the importance of these motifs for the the binding by OhrR.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|