Curation Information

BigR, a transcriptional repressor from plant-associated bacteria, regulates an operon implicated in biofilm growth.;Barbosa RL, Benedetti CE;Journal of bacteriology 2007 Sep; 189(17):6185-94 [17586627]
BigR [Q9PFB1, view regulon]
Reported TF sp.
Xylella fastidiosa 9a5c
Reported site sp.
Xylella fastidiosa 9a5c
Created by
Dinara Sagitova
Curation notes

Experimental Process

EMSA performed with a series of the blh promoter fragments determined that BigR binds to a region containing an imperfect palindrome. DNase I footprinting confirmed that BigR binds to this palindrome. blh-EGFP reporter assays showed BigR acts as a repressor of the blh gene. Site-directed mutagenesis in conjunction with EMSA verified that mutations in the BigR binding site affected BigR binding. Multiple sequence alignment of the blh promoters from X. fastidiosa, A. tumefaciens, S. meliloti, M. loti, Chromobacterium violaceum, and Nitrosomonas europaea identified the BigR consensus sequence.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... XF0768 XF0767 XF0766 XF0765 XF0764
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details GFP Promoter Fusion (ECO:0005636) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - repressor not specified