Curation Information

Publication
Binding of Pseudomonas aeruginosa AlgZ to sites upstream of the algZ promoter leads to repression of transcription.;Ramsey DM, Baynham PJ, Wozniak DJ;Journal of bacteriology 2005 Jul; 187(13):4430-43 [15968052]
TF
AmrZ [G3XCY4, view regulon]
Reported TF sp.
Pseudomonas aeruginosa FRD1
Reported site sp.
Pseudomonas aeruginosa FRD1
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

An algZ-lacZ fusion in the wild-type P. aeruginosa FRD1 and its algZ mutant demonstrated that AlgZ(AmrZ) acts as a autorepressor. EMSA performed with overlapping algZ promoter regions identified that ALgZ bind specifically to a region 66-185 bp upstream of the algZ transcription start site. By using EMSA and copper-phenanthroline footprinting, the authors identified that AlgZ binds at least two sites upstream of the algZ promoter and exhibits a different affinity for each site. Deletion of both binding sites resulted in the loss of AlgZ binding.

Transcription Factor Binding Sites


TGCCAGTACCAGCT
TGGCAAAACGCCGG
GGCCATCATCCAAC
TGCCAGTACCAGCT
TGGCAAAACGCCGG
GGCCATCATCCAAC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TGCCAGTACCAGCT amrZ,
... ... amrZ PA3372 PA3373 PA3374 PA3375 PA3376 PA3377 PA3378 PA3379 PA3380 PA3381 phnE PA3383 phnC
Experimental technique details Beta-gal reporter assay - Experimental technique details Copper-phenanthroline footprinting (ECO:0005626) - Experimental technique details EMSA (ECO:0001807) - repressor not specified
TGGCAAAACGCCGG amrZ,
... ... amrZ PA3372 PA3373 PA3374 PA3375 PA3376 PA3377 PA3378 PA3379 PA3380 PA3381 phnE PA3383 phnC
Experimental technique details Beta-gal reporter assay - Experimental technique details Copper-phenanthroline footprinting (ECO:0005626) - Experimental technique details EMSA (ECO:0001807) - repressor not specified
GGCCATCATCCAAC amrZ,
... ... amrZ phnC PA3383 phnE PA3381 PA3380 PA3379 PA3378 PA3377 PA3376 PA3375 PA3374 PA3373 PA3372
Experimental technique details Beta-gal reporter assay - Experimental technique details Copper-phenanthroline footprinting (ECO:0005626) - Experimental technique details EMSA (ECO:0001807) - repressor not specified