An algZ-lacZ fusion in the wild-type P. aeruginosa FRD1 and its algZ mutant demonstrated that AlgZ(AmrZ) acts as a autorepressor. EMSA performed with overlapping algZ promoter regions identified that ALgZ bind specifically to a region 66-185 bp upstream of the algZ transcription start site. By using EMSA and copper-phenanthroline footprinting, the authors identified that AlgZ binds at least two sites upstream of the algZ promoter and exhibits a different affinity for each site. Deletion of both binding sites resulted in the loss of AlgZ binding.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|