GUS reporter assays identified that the expression of nixA, nixC, nixD, nagK-IIA, and nagK-IIB was derepressed in the ΔnagR deletion mutant compared to the expression in the wild-type strain. The results of these assays were in agreement with the putative NagR boxes in the promoters of these genes. A new NagR consensus sequence (GTTGACARCGYTGTCANC) was identified by the authors based on the alignment of the four predicted NagR boxes. This NagR box differed at positions 1, 2, and 18 from the previously proposed NagR box (AATGACARCGYTGTCATT).
Quantitative data format: PSSM scoreGTTGACAACGCTGTCAAC 19.32
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|