Curation Information

Publication
The inter-kingdom solo OryR regulator of Xanthomonas oryzae is important for motility.;González JF, Myers MP, Venturi V;Molecular plant pathology 2013 Apr; 14(3):211-21 [23083431]
TF
OryR [Q5H3E9, view regulon]
Reported TF sp.
Xanthomonas oryzae pv. oryzae XKK.12
Reported site sp.
Xanthomonas oryzae pv. oryzae XKK.12
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

Microarray analysis established that in X. oryzae pv oryzae OryR regulated transcription of 330 genes by two-fold or more. qRT-PCR validated the microarray results in a set of 22 genes. The authors closely examined the influence of OryR on the flhF expression. Visual inspection of the flhF promoter identified a lux-box-like sequence. flhF-gus reporter assays in the WT and oryR mutant strains confirmed the OryR-dependent activation of flhF expression.

Transcription Factor Binding Sites


ACCTCATGACGCTGGCAACA
ACCTCATGACGCTGGCAACA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
ACCTCATGACGCTGGCAACA flhF,
... ... flhF fleN fliA cheY cheZ cheA XOO2625 tnpA XOO2627
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details GUS reporter gene assay (ECO:0005641) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details Visual sequence inspection (nan) - activator not specified