The objective of the authors was to systematically alter a TraR binding site (tra box) to all possible bases and determine the resulting affinity of the TraR to the altered sites. Site-directed mutagenesis was used to 27 tra box sequence variants. EMSA confirmed that TraR binds to the wild type tra box and determined the affinity of TraR to each mutant tra box. traI-lacZ fusion was used to test the effect of the tra box defects on transcription activation in vivo.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|