A consensus search found potential binding sites of CiaR in the S.mutans genome. DNase I footprinting determined a binding site at the ciaX gene. Site directed mutagenesis with EMSA confirmed the functionality proposed CiaR binding site. 5' RACE determined the transcription start site of the ciaX gene. DNA-array expression analysis determined that CiaR represses the ciaX gene.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|