Curation Information

Regulation of ciaXRH operon expression and identification of the CiaR regulon in Streptococcus mutans.;Wu C, Ayala EA, Downey JS, Merritt J, Goodman SD, Qi F;Journal of bacteriology 2010 Sep; 192(18):4669-79 [20639331]
CiaR [Q8DU28, view regulon]
Reported TF sp.
Streptococcus Mutans UA140
Reported site sp.
Streptococcus Mutans UA140
Created by
Joe Sparenberg
Curation notes

Experimental Process

A consensus search found potential binding sites of CiaR in the S.mutans genome. DNase I footprinting determined a binding site at the ciaX gene. Site directed mutagenesis with EMSA confirmed the functionality proposed CiaR binding site. 5' RACE determined the transcription start site of the ciaX gene. DNA-array expression analysis determined that CiaR represses the ciaX gene.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... SMU_1131c ciaR ciaH
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor not specified