lacZ assay results suggest that AraR represses the araABDLMNPQ-abfA operon. Site directed mutagenesis showed that mutations to the binding site increased expression of the araABDLMNPQ-abfA operon. in vitro transcription confirmed that AraR repressed the araABDLMNPQ-abfA operon. DNase I footprinting determined the binding sequence.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|