LuxT was identified as binding the smcR promoter through SDS-PAGE. EMSAs confirmed that it binds specifically and DNAse footprint identified a protected region with sequence AGTGCAATACGCTATTTACTATCACA. A luxT- mutant was shown to induce smcR expression through Western blot and luciferase reporter.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|