Visual inspection of the lacTEGF promoter identified a putative CcpA binding site with one mismatch to the CcpA consensus sequence. GUS reporter assays in the wild-type and ccpA mutant strains showed that repression by CcpA was abolished in the ccpA mutant strain.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|
|ATAAAACGTTTACA||lact, lacE, lacG, lacF,||