Northern blot analysis with mRNA obtained from the wild-type strain BL23 and ccpA mutant strain showed that pfk-pyk expression is repressed by CcpA. EMSA confirmed that CcpA bound to the pfk-pyk intergenic region. DNAse I footprinting showed that CcpA protected a region containing two degenerate CcpA motifs.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|