Curation Information

A key developmental regulator controls the synthesis of the antibiotic erythromycin in Saccharopolyspora erythraea.;Chng C, Lum AM, Vroom JA, Kao CM;Proceedings of the National Academy of Sciences of the United States of America 2008 Aug 12; 105(32):11346-51 [18685110]
BldD [A4FBF9, view regulon]
Reported TF sp.
Saccharopolyspora erythraea NRRL 2338
Reported site sp.
Saccharopolyspora erythraea NRRL 2338
Created by
Dinara Sagitova
Curation notes

Experimental Process

EMSA confirmed that BldD bound specifically to the promoter of the erythromycin biosynthetic gene cluster. DNase I footprinting localized BldD binding region in the eryBVI promoter. The authors identified a putative BldD binding site in this region based on the S.coelicolor BldD consensus, AGTgA-(n)m-TCACc. Deletion of bldD resulted in in the inability of the strain to form aerial mycelium and to sporulate on three different media. Complementation of the bldD deletion with a single copy of bldD restored the WT phenotype. Furthermore, bldD mutant produced 7-fold less erythromycin than the WT strain. Altogether these data allowed the authors to confirm BldD-mediated activation of the ery genes.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... eryBVI eryBVII eryCV eryCIV
Experimental technique details Ad-hoc qualitative phenotype observation (ECO:0005674) - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Visual sequence inspection (nan) - activator not specified