A CAT reporter assay determined the role of OhrR as an autorepressor. EMSA demonstrated binding of OhrR to the P1 promoter upstream of the ohrR transcriptional start site. DNase I footprintinglocalized the binding site in the promoter of interest. Site-directed mutagenesis of this site, in conjunction with CAT reporter assays further specified the identity of the OhrR binding site.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|