Curation Information

The repressor for an organic peroxide-inducible operon is uniquely regulated at multiple levels.;Mongkolsuk S, Panmanee W, Atichartpongkul S, Vattanaviboon P, Whangsuk W, Fuangthong M, Eiamphungporn W, Sukchawalit R, Utamapongchai S;Molecular microbiology 2002 May; 44(3):793-802 [11994159]
OhrR [M4TR54, view regulon]
Reported TF sp.
Xanthomonas campestris pv. phaseoli
Reported site sp.
Xanthomonas campestris pv. phaseoli
Created by
Grace Chandler
Curation notes

Experimental Process

A CAT reporter assay determined the role of OhrR as an autorepressor. EMSA demonstrated binding of OhrR to the P1 promoter upstream of the ohrR transcriptional start site. DNase I footprintinglocalized the binding site in the promoter of interest. Site-directed mutagenesis of this site, in conjunction with CAT reporter assays further specified the identity of the OhrR binding site.

Transcription Factor Binding Sites

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type