Curation Information

Publication
Substitution of glutamate residue by lysine in the dimerization domain affects DNA binding ability of HapR by inducing structural deformity in the DNA binding domain.;Singh R, Rathore YS, Singh NS, Peddada N, Ashish, Raychaudhuri S;PloS one 2013; 8(10):e76033 [24155884]
TF
HapR [A0A0H3Q915, view regulon]
Reported TF sp.
Vibrio cholerae C6706
Reported site sp.
Vibrio cholerae C6706
Created by
Erill Lab
Curation notes
-

Experimental Process

Previously reported site was confirmed with EMSA, both with w-t and mutant (does not bind) protein.

Transcription Factor Binding Sites


TTATTGAGAATAATGTCAGTTT
CTAATGATTATTTTGTTATTTG
TTATTGAGAATAATGTCAGTTT
CTAATGATTATTTTGTTATTTG

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TTATTGAGAATAATGTCAGTTT
... ... VCD_001716
Experimental technique details EMSA (ECO:0001807) - not specified not specified
CTAATGATTATTTTGTTATTTG
... ... VCD_001024 VCD_001025
Experimental technique details EMSA (ECO:0001807) - not specified not specified