Curation Information

Dual transcriptional control of amfTSBA, which regulates the onset of cellular differentiation in Streptomyces griseus.;Ueda K, Takano H, Nishimoto M, Inaba H, Beppu T;Journal of bacteriology 2005 Jan; 187(1):135-42 [15601697]
BldD [B1W457, view regulon]
Reported TF sp.
Streptomyces griseus subsp. griseus NBRC 13350
Reported site sp.
Streptomyces griseus subsp. griseus NBRC 13350
Created by
Dinara Sagitova
Curation notes

Experimental Process

The amfT transcription start site was identified by S1 protection assay. A sequence matching the BldD consensus binding site was identified in the amfT promoter. EMSA confirmed that BldD binds to the amfT promoter. S1 protection assays revealed that overproduction of bldD markedly repressed amfT promoter activity.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... amfT amfA amfB amfS
Experimental technique details EMSA (ECO:0001807) - Experimental technique details S1 nuclease protection (ECO:0005666) - Experimental technique details Visual sequence inspection (nan) - repressor not specified