EMSA showed that was able to bind 127 bp sigH promoter fragment. BldD binding site in the sigH promoter was further localized by performing EMSA with different length promoter probes. DNase I footprinting showed that BldD protected a 44 bp region in the sigH promoter. S1 nuclease protection analysis showed that sigH expression was significantly upregulated in the bldD mutant strain.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|