EMSA showed that BldD binds specifically to the whiG promoter region. DNase I footprinting identified that BldD protected two sites in the whiG promoter. S1 nuclease assay experiment compared the expression levels of whiG in the wild type and bldD mutant strains and the results suggested that BldD repressed whiG expression. Northern blot analysis confirmed BldD-mediated regulation. Similarly, BldD-mediated regulation of bldN and bdtA was shown.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|