Curation Information

The Vibrio cholerae fatty acid regulatory protein, FadR, represses transcription of plsB, the gene encoding the first enzyme of membrane phospholipid biosynthesis.;Feng Y, Cronan JE;Molecular microbiology 2011 Aug; 81(4):1020-33 [21771112]
FadR [A5F6Z2, view regulon]
Reported TF sp.
Vibrio cholerae O395
Reported site sp.
Vibrio cholerae O395
Created by
Dinara Sagitova
Curation notes

Experimental Process

plsB transcriptional start site was determined by RLM-RACE. Inspection of the promoter sequence identified a putative FadR binding site. EMSA confirmed that FadR binds to a 39 bp probe containing the putative 17 bp FadR binding site. Binding was reversed upon addition of long-chain acyl-CoA. plsB-lacZ promoter assays performed in E.coli showed that plsB expression was 2.5-fold higher in the fadR mutant. plsB-lacZ fusion in V.cholerae showed that FadR-mediated repression was abolished when oleic acid was supplemented to the RB medium.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... plsB lexA VC0395_A2424 dinF
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Visual sequence inspection (nan) - repressor not specified