plsB transcriptional start site was determined by RLM-RACE. Inspection of the promoter sequence identified a putative FadR binding site. EMSA confirmed that FadR binds to a 39 bp probe containing the putative 17 bp FadR binding site. Binding was reversed upon addition of long-chain acyl-CoA. plsB-lacZ promoter assays performed in E.coli showed that plsB expression was 2.5-fold higher in the fadR mutant. plsB-lacZ fusion in V.cholerae showed that FadR-mediated repression was abolished when oleic acid was supplemented to the RB medium.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|