Curation Information

Identification of a distantly located regulatory element in the luxD gene required for negative autoregulation of the Vibrio fischeri luxR gene.;Shadel GS, Baldwin TO;The Journal of biological chemistry 1992 Apr 15; 267(11):7690-5 [1560004]
LuxR [B5EV73, view regulon]
Reported TF sp.
Vibrio fischeri MJ11
Reported site sp.
Vibrio fischeri MJ11
Created by
Dinara Sagitova
Curation notes

Experimental Process

A deletion analysis of the luxICD region identified LuxR binding region. Visual inspection of this 56 bp region identified a 20 bp sequence similar to the lux consensus. Deletion of this site by site-directed mutagenesis resulted in increased expression of luxR compared to the expression levels in the strain containing the wild type operator. The ability of luxD element to bind LuxR was demonstrated by the ability of LuxR to activate luxICDABEG operon transcription from a luxICDABEG operon reporter plasmid in which the wild-type lux operator was replaced with the luxD element. In the presence of autoinducer, LuxR was able to activate luxI operon transcription from this luxD element containing reporter vector.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... luxD VFMJ11_A1039 VFMJ11_A1038 VFMJ11_A1037 fre
Experimental technique details Ad-hoc quantitative phenotype observation (ECO:0005676) - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Visual sequence inspection (nan) - repressor not specified