A deletion analysis of the luxICD region identified LuxR binding region. Visual inspection of this 56 bp region identified a 20 bp sequence similar to the lux consensus. Deletion of this site by site-directed mutagenesis resulted in increased expression of luxR compared to the expression levels in the strain containing the wild type operator. The ability of luxD element to bind LuxR was demonstrated by the ability of LuxR to activate luxICDABEG operon transcription from a luxICDABEG operon reporter plasmid in which the wild-type lux operator was replaced with the luxD element. In the presence of autoinducer, LuxR was able to activate luxI operon transcription from this luxD element containing reporter vector.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|