Curation Information

Publication

Cooperative regulation of the Vibrio vulnificus nan gene cluster by NanR protein, cAMP receptor protein, and N-acetylmannosamine 6-phosphate.;Kim BS, Hwang J, Kim MH, Choi SH;The Journal of biological chemistry 2011 Nov 25; 286(47):40889-99 [21956110]

TF

NanR [Q7MD38, view regulon]

Reported TF sp.

Vibrio vulnificus ATCC 29307

Reported site sp.

Vibrio vulnificus ATCC 29307

Created by

Dinara Sagitova

Curation notes

less than 90% of reported sites are located as exact matches.

Experimental Process

The transcription start sites of nanT and nanEK nagA were determined by primer extension analyses. Comparison of the expression levels of the wild-type and nanR mutant strains by qRT-PCR revealed that NanR repressed nanT and nanE expression. EMSA showed that NanR bound specifically to the nanT-nanE intergenic region. DNase I footprinting revealed that NanR protected a region containing a 19 bp inverted repeat.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.