The transcription start sites of nanT and nanEK nagA were determined by primer extension analyses. Comparison of the expression levels of the wild-type and nanR mutant strains by qRT-PCR revealed that NanR repressed nanT and nanE expression. EMSA showed that NanR bound specifically to the nanT-nanE intergenic region. DNase I footprinting revealed that NanR protected a region containing a 19 bp inverted repeat.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|