Curation Information

Bioluminescence in Vibrio fischeri is controlled by the redox-responsive regulator ArcA.;Bose JL, Kim U, Bartkowski W, Gunsalus RP, Overley AM, Lyell NL, Visick KL, Stabb EV;Molecular microbiology 2007 Jul; 65(2):538-53 [17590235]
ArcA [Q5E2Y1, view regulon]
Reported TF sp.
Vibrio fischeri ES114
Reported site sp.
Vibrio fischeri ES114
Created by
Dinara Sagitova
Curation notes

Experimental Process

GFP promoter fusion experiments showed that luxI promoter was derepressed in the arcA mutant compared to the wild-type V. fischeri ES114 strain. EMSA using E.coli ArcA confirmed binding to the lux promoter region. DNase I footprinting revealed that ArcA protected two sites (site 1 and site 2) in the lux promoter fragment. Deletion of site 1 resulted in an increase in luminescence equivalent to that observed in a arcA mutant strain. Multiple sequence alignment showed that this sequence was conserved in the lux promoters of strains MJ1, ES114 and ATCC7744.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... luxI luxC luxD luxA luxB luxE fre luxR
Experimental technique details Ad-hoc quantitative phenotype observation (ECO:0005676) - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details GFP Promoter Fusion (ECO:0005636) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - repressor not specified