GFP promoter fusion experiments showed that luxI promoter was derepressed in the arcA mutant compared to the wild-type V. fischeri ES114 strain. EMSA using E.coli ArcA confirmed binding to the lux promoter region. DNase I footprinting revealed that ArcA protected two sites (site 1 and site 2) in the lux promoter fragment. Deletion of site 1 resulted in an increase in luminescence equivalent to that observed in a arcA mutant strain. Multiple sequence alignment showed that this sequence was conserved in the lux promoters of strains MJ1, ES114 and ATCC7744.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|