Curation Information

Insights into the LexA regulon of Thermotogales.;Mazón G, Campoy S, Fernández de Henestrosa AR, Barbé J;Antonie van Leeuwenhoek 2006 Aug; 90(2):123-37 [16897562]
LexA [A9BGA3, view regulon]
Reported TF sp.
Petrotoga miotherma
Reported site sp.
Petrotoga miotherma
Created by
Erill Lab
Curation notes
Data originally reported for P. miotherma. Genome still in progress and not likely to be completed soon.

Experimental Process

EMSA identifies binding of purified LexA in the upstream region of its operon (metS-gyrA-lexA; RT-PCR). Footprinting reveals protected 19 bp fragment. Site directed mutagenesis with EMSAs shows that a palindromic region within footprint is required for binding. Further EMSA on uvrA promoter confirms that site located with PSSM search is also bound.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... Pmob_1239 Pmob_1238 Pmob_1237 Pmob_1236 Pmob_1235 Pmob_1234 Pmob_1233 Pmob_1232 Pmob_1231
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - not specified dimer
... ... uvrA
Experimental technique details EMSA (ECO:0001807) - Experimental technique details PSSM site search (ECO:0005659) - not specified dimer