EMSA identifies binding of purified LexA in the upstream region of its operon (metS-gyrA-lexA; RT-PCR). Footprinting reveals protected 19 bp fragment. Site directed mutagenesis with EMSAs shows that a palindromic region within footprint is required for binding. Further EMSA on uvrA promoter confirms that site located with PSSM search is also bound.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|