Microarray analyses identified AphA-regulated genes by comparing expression profile of a luxO-D47E luxR- strain to that of a luxO-D47E luxR-aphA- strain. qRT-PCR performed in wild-type and AphA mutant strains showed that AphA repressed qrr4 expression. EMSA confirmed that AphA binds to the qrr4 promoter. LuxR binding site was identified in a different study (PMID: 18681939).
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|