Curation Information

Individual and combined roles of the master regulators AphA and LuxR in control of the Vibrio harveyi quorum-sensing regulon.;van Kessel JC, Rutherford ST, Shao Y, Utria AF, Bassler BL;Journal of bacteriology 2013 Feb; 195(3):436-43 [23204455]
AphA [A7MXA8, view regulon]
Reported TF sp.
Vibrio campbellii ATCC BAA-1116
Reported site sp.
Vibrio campbellii ATCC BAA-1116
Created by
Dinara Sagitova
Curation notes

Experimental Process

Microarray analyses identified AphA-regulated genes by comparing expression profile of a luxO-D47E luxR- strain to that of a luxO-D47E luxR-aphA- strain. qRT-PCR performed in wild-type and AphA mutant strains showed that AphA repressed qrr4 expression. EMSA confirmed that AphA binds to the qrr4 promoter. LuxR binding site was identified in a different study (PMID: 18681939).

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... VIBHAR_06697 VIBHAR_06698
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor not specified