Comparison of the rtxA1-lacZ expression levels in the hns+ and hns- strains revealed that H-NS negatively regulates rtxA1 expression. EMSA determined that up to 0.7 uM of H-NS had to be added before binding was observed. DNase I footprinting verified this result by showing that binding was not observed until 0.8 uM of H-NS was added.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|