Curation Information

Publication
Expression of the cpdA gene, encoding a 3',5'-cyclic AMP (cAMP) phosphodiesterase, is positively regulated by the cAMP-cAMP receptor protein complex.;Kim HS, Kim SM, Lee HJ, Park SJ, Lee KH;Journal of bacteriology 2009 Feb; 191(3):922-30 [19028903]
TF
CRP [Q7M7I9, view regulon]
Reported TF sp.
Vibrio vulnificus ATCC 29307
Reported site sp.
Vibrio vulnificus ATCC 29307
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

The transcription start site for the mutT-yqiB-cpdA-yqiA operon was determined by primer extension. Visual inspection identified the presence of the CRP consensus in the cpdA promoter region. cpdA-luxAB transcriptional fusion showed that cdpA expression decreased in the crp mutant strain compared to the wild-type strain. EMSA showed that CRP bound specifically to the cpdA promoter. Site-directed mutagenesis of the CRP-binding site in conjunction with EMSA confirmed that cpdA expression is activated by cAMP-CRP acting on the region between positions −106 and −85 relative to its transcription start site.

Transcription Factor Binding Sites


AGTTGTGCAATAAATGAATTGT
AGTTGTGCAATAAATGAATTGT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
AGTTGTGCAATAAATGAATTGT VV0584, VV0585, VV0586, VV0587,
... ... VV0584 VV0585 VV0586 VV0587 tolC
Experimental technique details EMSA (ECO:0001807) - Experimental technique details Luciferase reporter assay (ECO:0005648) - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Visual sequence inspection (nan) - activator not specified