The transcription start site for the mutT-yqiB-cpdA-yqiA operon was determined by primer extension. Visual inspection identified the presence of the CRP consensus in the cpdA promoter region. cpdA-luxAB transcriptional fusion showed that cdpA expression decreased in the crp mutant strain compared to the wild-type strain. EMSA showed that CRP bound specifically to the cpdA promoter. Site-directed mutagenesis of the CRP-binding site in conjunction with EMSA confirmed that cpdA expression is activated by cAMP-CRP acting on the region between positions −106 and −85 relative to its transcription start site.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|
|AGTTGTGCAATAAATGAATTGT||VV0584, VV0585, VV0586, VV0587,||