Northern blot analysis showed that vvhBA expression was reduced in the crp mutant compared to the wild-type strain. RNA dot blot experiment confirmed that CRP acted as a positive regulator of vvhBA. vvhBA transcription start site was determined by a primer extension analysis. Deletion analysis of the vvhBA promoter region in conjunction with the luxAB reporter assays identified the region in the vvhBA promoter containing the CRP binding site. EMSA confirmed that CRP bound specifically to the vvhBA promoter. DNase I footprinting showed that CRP protected a region in the vvhBA promoter that contained a CRP consensus sequence.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|