Curation Information

Promoter analysis and regulatory characteristics of vvhBA encoding cytolytic hemolysin of Vibrio vulnificus.;Choi HK, Park NY, Kim DI, Chung HJ, Ryu S, Choi SH;The Journal of biological chemistry 2002 Dec 6; 277(49):47292-9 [12356775]
CRP [Q7M7I9, view regulon]
Reported TF sp.
Vibrio vulnificus ATCC 29307
Reported site sp.
Vibrio vulnificus ATCC 29307
Created by
Dinara Sagitova
Curation notes

Experimental Process

Northern blot analysis showed that vvhBA expression was reduced in the crp mutant compared to the wild-type strain. RNA dot blot experiment confirmed that CRP acted as a positive regulator of vvhBA. vvhBA transcription start site was determined by a primer extension analysis. Deletion analysis of the vvhBA promoter region in conjunction with the luxAB reporter assays identified the region in the vvhBA promoter containing the CRP binding site. EMSA confirmed that CRP bound specifically to the vvhBA promoter. DNase I footprinting showed that CRP protected a region in the vvhBA promoter that contained a CRP consensus sequence.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... VVA0964 VVA0965 VVA0963
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Luciferase reporter assay (ECO:0005648) - Experimental technique details Northern blot (ECO:0005653) - Experimental technique details RNA dot blot (ECO:0001177) - activator not specified