Curation Information

Regulation of the Vibrio vulnificus hupA gene by temperature alteration and cyclic AMP receptor protein and evaluation of its role in virulence.;Oh MH, Lee SM, Lee DH, Choi SH;Infection and immunity 2009 Mar; 77(3):1208-15 [19139193]
CRP [Q7M7I9, view regulon]
Reported TF sp.
Vibrio vulnificus ATCC 29307
Reported site sp.
Vibrio vulnificus ATCC 29307
Created by
Dinara Sagitova
Curation notes

Experimental Process

Quantitative real-time PCR showed that the level of hupA transcript was reduced in the crp mutant compared to the wild-type strain. Primer extension analysis performed using the wild-type and the crp mutant strains confirmed that the expression of hupA in V. vulnificus is under the positive control of CRP. EMSA confirmed that CRP activates hupA expression by directly binding to the hupA promoter. DNase I footprinting showed that CRP protected a region containing a consensus CRP binding site.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... VVA0781 VVA0782
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Primer Extension assay (ECO:0005657) - Experimental technique details qPCR [quantitative real-time] (ECO:0005660) - activator not specified