vvsA-luxAB reporter fusion showed that vvsA expression repressed by Fur in the presence of iron. Visual inspection of the vvsA promoter identified a Fur box with only two nucleotide differences from the consensus Fur binding site. EMSA showed that Fur bound specifically to the vvsA promoter. DNase I footprinting identified that Fur binding region contained the putative Fur box.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|