EMSA of arcR with an upstream region of the arc promoter demonstrated specific binding, improved by arginine. Additional site-deleted probes indicated only one binding site, but low-level residual binding suggested one or more low-affinity bindings sites as well. DNase I protection was used to identify the binding site. CAT assays with glucose and galactose indicated the arc operon is subject to CCR, and partial-deletion mutant strains of the binding site demonstrated the entire protected region to be necessary for binding.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|