qRT-PCR validated the cotranscription of cydA and cydB. LacZ fusions using truncated promoter regions measured cydA-lacZ expression, identifying a critical region for transcription upstream of the start site. Visual inspection showed this region contained a 10bp inverted repeat and a motif similar to the CMP binding motif. Site-directed mutagenesis in conjunction with lacZ fusion assays verified the proposed binding sequence.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|