Curation Information

Hypoxia-activated cytochrome bd expression in Mycobacterium smegmatis is cAMP receptor protein (CRP)-dependent.;Aung HL, Berney M, Cook GM;Journal of bacteriology 2014 Jun 16; (): [24936051]
CRP [A0R5H1, view regulon]
Reported TF sp.
Mycobacterium smegmatis str. MC2 155
Reported site sp.
Mycobacterium smegmatis str. MC2 155
Created by
Grace Chandler
Curation notes

Experimental Process

qRT-PCR validated the cotranscription of cydA and cydB. LacZ fusions using truncated promoter regions measured cydA-lacZ expression, identifying a critical region for transcription upstream of the start site. Visual inspection showed this region contained a 10bp inverted repeat and a motif similar to the CMP binding motif. Site-directed mutagenesis in conjunction with lacZ fusion assays verified the proposed binding sequence.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... MSMEG_3233 cydB MSMEG_3234 cydD MSMEG_3230 MSMEG_3229 MSMEG_3235 MSMEG_3236 MSMEG_3237 MSMEG_3238 MSMEG_3239 MSMEG_3240 MSMEG_3241 MSMEG_3242
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Visual sequence inspection (nan) - activator not specified