Curation Information

Publication
CcpA mediates the catabolite repression of tst in Staphylococcus aureus.;Seidl K, Bischoff M, Berger-B├Ąchi B;Infection and immunity 2008 Nov; 76(11):5093-9 [18710862]
TF
CcpA [Q2G295, view regulon]
Reported TF sp.
Staphylococcus aureus subsp. aureus N315
Reported site sp.
Staphylococcus aureus subsp. aureus N315
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

A putative CcpA binding site was identified in the tst promoter region by screening S. aureus N315 with the CcpA consensus sequence. Multiple sequence alignment confirmed that this sequence was conserved in tst promoters of the strains N315 and RF122. Luciferase reporter assays identified that glucose-dependent repression of tst transcription required CcpA.

Transcription Factor Binding Sites


TATGTAAACGTTTACACA
TATGTAAACGTTTACACA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TATGTAAACGTTTACACA tst
... ... tst
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details Luciferase reporter assay (ECO:0005648) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - repressor not specified