Curation Information

Elaborate transcription regulation of the Bacillus subtilis ilv-leu operon involved in the biosynthesis of branched-chain amino acids through global regulators of CcpA, CodY and TnrA.;Tojo S, Satomura T, Morisaki K, Deutscher J, Hirooka K, Fujita Y;Molecular microbiology 2005 Jun; 56(6):1560-73 [15916606]
CcpA [P25144, view regulon]
Reported TF sp.
Bacillus subtilis subsp. subtilis str. 168
Reported site sp.
Bacillus subtilis subsp. subtilis str. 168
Created by
Dinara Sagitova
Curation notes

Experimental Process

LacZ reporter assays showed that ilv-leu was under CcpA-dependent positive control. GRASP-DNA program revealed a CcpA binding motif in the ilv-leu promoter. Site-directed mutagenesis was used to introduce mutations into the putative CcpA binding site. DNase I footprinting with probes containing wild type and mutated CcpA binding site showed that the CcpA binding site was protected from digestion by DNase only in the wild type promoter.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... ilvB ilvH ilvC BSU_misc_RNA_45 leuA leuB leuC leuD ysoA ysnD
Experimental technique details Beta-gal reporter assay - Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details Site directed mutagenesis (ECO:0005667) - activator not specified