LacZ reporter assays showed that ilv-leu was under CcpA-dependent positive control. GRASP-DNA program revealed a CcpA binding motif in the ilv-leu promoter. Site-directed mutagenesis was used to introduce mutations into the putative CcpA binding site. DNase I footprinting with probes containing wild type and mutated CcpA binding site showed that the CcpA binding site was protected from digestion by DNase only in the wild type promoter.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|
|ATGAAAGCGTATACA||ilvB, ilvH, ilvC,||